With emergence of near infrared spectroscopy nirs as a. Infrared spectroscopy, principle, instrumentation and. Nearinfrared spectroscopy in food analysis hackteria. There are three well defined ir regions near, mid and far. Introduction to infrared spectroscopy chromacademy. The light beam split into two by the beam splitter is reflected from the moving. In the last ten years, near infrared spectroscopy nirs has become a very popular technique for a wide. Principles of ftir spectroscopy in ftir analyses, infrared light from the light source passes through a michelson interferometer along the optical path. Near infra red spectroscopy an overview international journal. Nearinfrared spectroscopyits versatility in analytical chemistry. Infrared spectroscopy involves the interaction of infrared radiation with matter. With emergence of nearinfrared spectroscopy nirs as a fast and non destructive analytical method, researchers have used nirs for.
Principles of ftir spectroscopy michigan state university. With emergence of near infrared spectroscopy nirs as a fast. Introduction the investigation of characteristics of near infrared nir region of the electromagnetic spectrum starts with herschel in 1800 herschel 1800. The energies are reliant on the shape of the molecular surfaces, the associated vibronic coupling, and the mass corresponding to. Nearinfrared spectroscopy measures the absorption of electromagnetic radiation including wavelengths from 750 to 2500 nm. On the other hand, the presence of overtones makes nir. Origin and characteristics of nir absorption bands. Nir instrumentation is simple to operate and no chemicals are used or generated andrade 2007. Pdf principles, techniques, and limitations of near. The concentrations of constituents such as water, protein, fat and carbohydrate can in principle be determined using classical absorption spectroscopy. The american society of testing and materials astm defines the nir region of the electromagnetic spectrum as the wavelength range of 7802526 nm corresponding to the wave number range 128203959 cm. Principles, techniques, and limitations of near infrared spectroscopy. Near infrared nir spectroscopy and imaging are fast and nondestructive analytical techniques that provide chemical and physical information of virtually any matrix. Basic principles of near infrared nir spectroscopy 2.
The continuous preference of the oral route for the delivery of the drugs has led the ongoing research in the field of oral delivery. The ir spectroscopy theory utilizes the concept that molecules tend to absorb specific frequencies of light that are characteristic of the corresponding structure of the molecules. In combination with multivariate data analysis these two methods open many interesting perspectives for both qualitative and quantitative analysis. The higherenergy nearir, approximately 140004000 cm. Underlying principal of infrared spectroscopy is based upon the molecular vibration which is further composed of the stretching and the bending vibration of a molecule. Principles, techniques, and limitations of near infrared. The michelson interferometer comprises a beam splitter, moving mirror, and fixed mirror.
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